Intended use: For the quantitative determination of total L-homocysteine in human citrated plasma on IL Coagulation systems.
Principle: Homocysteine (Hcy) levels in patient plasma are measured automatically on IL Coagulation systems in three stages:
- Reduction of mixed disulfides and protein-bound forms of Hcy present in the plasma samples to free Hcy
- Enzymatic conversion of free Hcy to S-adenosyl-L-Hcy (SAH) by the SAH hydrolase (SAHH) in the presence of an excess of adenosine
- Competitive agglutination reaction between anti-SAH and SAH/conjugate
Vials and volumes:
- 2 x 2mL a-SAH Latex Reagent (lyo)
- 2 x 2 mL Reductant (liq)
- 2 x 2 mL Enzyme (liq)
- 2 x 2.5 mL Conjugate (liq)
- 2 x 9 mL Buffer (liq)
- 2 x 1 mL Calibrator (liq)
*ACL, ACL TOP, and HemosIL are trademarks of Instrumentation Laboratory.