CEQ 8000 delivers precise DNA sizing, accurate allele alignment and automated data review.
Whether you are mapping "inborn error of metabolism," genotyping an individual, or quantifying microsatellite instability, the accurate and timely assignment of alleles can dramatically impact your lab's productivity. Beckman Coulter developed CEQTM 8000 with these processes in mind by providing high-precision DNA sizing, with sophisticated software algorithms. You can identify alleles even in the presence of enzyme stutter and the plus A artifact. The CEQ software automatically assesses quality and highlights results that need further review, allowing you to rapidly assess and interpret your data.
Data from the CEQ 8000 highlights the precision of the system for the analysis of microsatellite amplification products in the range of 200, 300 and 400 with a standard deviation < 0.27 bases, regardless of which capillary within an array, run gel lot or instrument used to separate the sample.
|
Experiment
|
|
Expected Allele
Size (bases)
Observed Sizes
|
196
|
277
|
281
|
403
|
|
1
2
3
|
Instrument 1
Instrument 2
Instrument 3
|
Mean
S.D.
Mean
S.D.
Mean
S.D.
|
196.16
0.04
196.21
0.05
196.19
0.03
|
278.53
0.12
278.86
0.13
278.40
0.13
|
282.62
0.10
282.94
0.13
282.48
0.13
|
402.45
0.11
402.69
0.14
402.28
0.09
|
| |
Across
Instruments
|
Mean
S.D.
|
196.19
0.05
|
278.59
0.24
|
282.68
0.24
|
402.47
0.21
|
|
1
2
3
|
Array 1
Array 2
Array 3
|
Mean
S.D.
Mean
S.D.
Mean
S.D.
|
196.19
0.03
196.14
0.05
196.07
0.05
|
278.40
0.13
278.67
0.13
278.69
0.12
|
282.48
0.13
282.74
0.12
282.77
0.14
|
402.28
0.09
402.49
0.11
402.45
0.13
|
| |
Across
Arrays
|
Mean
S.D.
|
196.13
0.18
|
278.58
0.19
|
282.66
0.19
|
402.40
0.15
|
|
1
2
3
|
Gel 1
Gel 2
Gel 3
|
Mean
S.D.
Mean
S.D.
Mean
S.D.
|
196.07
0.06
195.95
0.03
195.91
0.03
|
278.69
0.12
278.46
0.09
278.50
0.06
|
282.77
0.12
282.53
0.09
282.57
0.06
|
402.45
0.13
401.98
0.10
401.95
0.11
|
| |
Across
Gels
|
Mean
S.D.
|
195.98
0.08
|
278.54
0.15
|
282.62
0.15
|
402.13
0.27
|
| |
Across All
Variables
|
Mean
S.D.
|
196.10
0.12
|
278.57
0.20
|
282.65
0.20
|
402.33
0.26
|
|
