The WellRED fluorescent dyes from
Beckman Coulter facilitate high sensitivity detection. Excitation of
these dyes is achieved with a 650 nm and 750 nm diode laser. These
diode lasers have a long life and are inexpensive to maintain. The
emissions from these dyes are well separated and are in the infrared
when there is less interference from biological materials. The
WellRED dyes are available as dideoxy terminators, ddA, ddC, ddG,
and ddT within the CEQ™ Dye Terminator Cycle Sequencing (DTCS) Kits,
CEQ SNP-Primer Extension Kit, or may be purchased as dye-labeled
custom oligonucleotides from Proligo.
Additionally, the WellRED Dye-Labeled Phosphoramidites are
easily coupled to the 5' end of oligonucleotides using
commercial DNA synthesizers.
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