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From tissues to targets
Compared to traditional fractionation techniques, advantages of our chromatographic approach include: high loading capacity without band distortion; improved detection of low-abundance species, membrane or hydrophobic proteins, and low molecular weight proteins; enhanced reproducibility; a contamination-free liquid flow path; simple automation; and liquid fractions.
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Product Features
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The advantages are easy to see
By providing an automated, two-dimensional fractionation system expressly designed for high-resolution analysis of complex protein mixtures, the ProteomeLab PF 2D delivers an integrated solution that effectively resolves the thousands of proteins present in cell lysates.
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Since the ProteomeLab PF 2D system generates data from two dimensions, detailed protein maps can be constructed for easy comparison using the ProteomeLab Software Suite. Liquid fractions can be collected and stored for future analysis or the eluent can be connected directly to ESI-mass spectrometry.
The PF 2D's unique combination of chromatofocusing followed by non-porous reverse phase chromatography provides ultra-high resolution of proteins, while delivering information that can be related to 2D gel data.
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High-Resolution Capability
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The ProteomeLab PF 2D's second dimension is capable of resolving hundreds of proteins in great detail from each first-dimension fraction. A close up view of Fraction 16 is shown, illustrating high resolution separation from Mouse Embryotic Stem Cell Lysate figure. |
Clear Visualization of Results
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ProteomeLab software suite generates high resolution, color-coded protein maps that are easy to read and interpret. |
More Consistent Results
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The high precision of chromatographic techniques--combined with our world-class pump technology--delivers significantly greater reproducibility than traditional gel methods. |
Enhanced Quantitation
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Compared to staining and densitometry, our refined electronic peak-detection algorithms provide much more accurate integration.
| Pk # |
Retention Time |
Height |
Area |
Area Percent |
| 1 |
11.517 |
2130 |
11658 |
0.133 |
| 2 |
14.817 |
88390 |
304027 |
3.480 |
| 3 |
15.133 |
6718 |
64922 |
0.743 |
| 4 |
15.350 |
23078 |
153670 |
1.759 |
| 5 |
15.492 |
60375 |
269534 |
3.085 |
| 6 |
15.675 |
20819 |
101978 |
1.167 |
| 7 |
15.800 |
9481 |
65090 |
0.745 |
| 8 |
15.983 |
7419 |
41820 |
.0479 |
| 9 |
16.242 |
86094 |
385635 |
4.414 |
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Easy Automation, for Efficient Workflow
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Fraction collection from the first dimension is quality-controlled with an in-line pH monitor, then automatically injected into the second dimension. As a result, PF 2D requires much less time and attention than traditional labor-intensive techniques. |
Convenient MS compatibility
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Liquid fractions can be stored or transferred to a MALDI plate spotter, or directly connected to an electrospray source. |
Clean and controlled sample handling
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The ProteomeLab PF 2D's closed system utilizes a self-contained liquid flow path, reducing the sample contamination and transfer loss so commonly seen with manual systems. |
Extended Coverage
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The ProteomeLab PF 2D Kit contains the buffers and columns needed for 2D protein fractionation, and is optimized for use with the PF 2D system. |
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Proteins can be found over a wide range of molecular weight designations, including those below 20,000 KD. |
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![eLabNotebook[TM]](/products/images/analytical/protein/pf2d_eln.jpg)
