Automation of 1-10 Plates of a Beckman-Coulter-Immunotech Cytokine ELISA for IL-8 on the Biomek 2000 Workstation - Application to the Beckman-Coulter-Immunotech ELISA for IL-8
V. Rocchi, Ph. Denizot and J. Fieschi
Immunoanalysis Department, Research & Development
Immunotech, a Beckman Coulter Company
Biomek 2000 Equipment
| Source | Part No. | Quantity | Description |
| Beckman Coulter, Inc. | 609000 | 1 | Biomek 2000 Workstation |
| Beckman Coulter, Inc. | 609047 | 1 | Right Side Module |
| Beckman Coulter, Inc. | 267653 | 1 | Controller with BioWorks™ |
| Beckman Coulter, Inc. | 609001 | 1 | Gripper Tool with Disposal Option |
| Beckman Coulter, Inc. | 609056 | 1 | Wash Unit with 6-Port Valve |
| Beckman Coulter, Inc. | 267645 | 1 | Right Side Stacker Carousel System |
| Beckman Coulter, Inc. | 609120 | 4 | Labware Holder |
| Beckman Coulter, Inc. | 609027 | 1 | Eight Channel Wash Tool |
| Beckman Coulter, Inc. | 609025 | 1 | Eight Channel MP200 Tool |
| Beckman Coulter, Inc. | 609022 | 1 | Single Channel P200L Tool |
| Beckman Coulter, Inc. | 372795 | 1 | Reservoir Frame |
| Beckman Coulter, Inc. | 609055 | 1 | Plate Reader Worksurface Mounting Kit |
Consumables and Reagents
| Source | Part No. | Quantity | Description |
| Beckman Coulter, Inc. | IM2237 OR |
10 | Cytokine IL8 ELISA Kit |
| Beckman Coulter, Inc. | IM2238 | 2 | IL-8 ELISA Jumbo Kit (5 plates) |
| Beckman Coulter, Inc. | 372784 | 1 | Full Reservoir |
| Beckman Coulter, Inc. | 372786 | 3 | Half Reservoir |
| Beckman Coulter, Inc. | 372654 | 2 | Case of P250 Nonsterile Tips |
| Beckman Coulter, Inc. | 140504 | 1 | Beckman Square Well Titer Plate 25/case |
Suitable Vacuum Pumps
| Source |
Part No. |
Quantity | Description |
| Gast mfg. | 0523U4FG582DX | 1 | Rotary Oil Vacuum Pump 115VAC 60Hz |
| VWR Scientific | 0323V4AG582DX | 1 | Rotary Oil Vacuum Pump 115VAC 60Hz |
| VWR Scientific | 0323V4AG21DX | 1 | Rotary Oil Vacuum Pump 115VAC 60Hz |
| Cole Parmer | P-07055-04 | 1 | Rotary Oil Vacuum Pump 115VAC 60Hz |
| Cole Parmer | P-07055-35 | 1 | Rotary Oil Vacuum Pump 115VAC 60Hz |
Suitable Vacuum Bottles and Cap Pairings
| Source | Part No. | Quantity | Description |
| Beckman Coulter, Inc. | 975796 | 1 | Heavy-duty Bottle, HDPP 4 Liter |
| Beckman Coulter, Inc. | 975797 | 1 | CAP Filling/Venting 1/2" fittings, PP |
| VWR Scientific | 16331-100 | 1 | Heavy-duty Bottle, HDPP 4 Liter |
| VWR Scientific | 16225-230 | 1 | Filling/Venting CAP 1/2" fittings, PP |
| Nalgene | 2126-4000 | 1 | Heavy-duty Bottle, HDPP 4 Liter |
| Nalgene | 2162-0830 | 1 | Filling/Venting Closure 1/2" fittings, PP |
| Cole Parmer | P-06258-00 | 1 | Filling/Venting CAP 1/2" fittings, PP |
- Other Material required, but not provided:
Microplate reader with a 450nm filter; The Molecular devices Vmax* Microplate reader with Softmax can be directly controlled from the method.
* All trademarks are the property of their respective owners. Where applicable, the PCR process is covered by patents owned by Roche Molecular Systems, Inc., and F. Hoffman-LaRoche, Ltd.
