eLabNotebook > Bioassays > Cytokine ELISA 1-3 Plates Biomek® 2000

Automation of 1-3 Plates of a Beckman Coulter-Immunotech Cytokine ELISA for IL-8 on the Biomek® 2000 Workstation

V. Rocchi, Ph. Denizot  and J. Fieschi
Immunoanalysis Department, Research & Development
Immunotech, a Beckman Coulter Company

Biomek 2000 Equipment

Source Part No. Quantity Description
Beckman Coulter, Inc. 609000 1 Biomek 2000 Workstation
Beckman Coulter, Inc. 609047 1 Right Side Module
Beckman Coulter, Inc. 609048 1 Left Side Module
Beckman Coulter, Inc. 609056 1 Wash Unit with 6-port Valve
Beckman Coulter, Inc. 609120 5 Labware Holder
Beckman Coulter, Inc. 609121 1 Tip Rack Holder
Beckman Coulter, Inc. 372795 1 Reservoir Holder
Beckman Coulter, Inc. 609027 1 Wash-8 Tool
Beckman Coulter, Inc. 609025 1 MP200 200µL Multichannel Tool
Beckman Coulter, Inc. 609022 1 P200L 200µL Single Channel Tool

Consumables and Reagents

Source Part No. Quantity Description
Beckman Coulter, Inc. 372790 2 Quarter Reservoir
Beckman Coulter, Inc. 372788 2 Quarter Reservoir Divided by Length
Beckman Coulter, Inc. 372654 1  Case of P250 Nonsterile Tips 
Beckman Coulter, Inc. 140504 1 Beckman Square Well Titer Plate 25/case
Beckman Coulter, Inc. IM2237 1 Cytokine IL8 ELISA Kit

Suitable Vacuum Pumps

Source

Part No.

Quantity Description
Gast mfg. 0523U4FG582DX 1 Rotary Oil Vacuum Pump 115VAC 60Hz
VWR Scientific 0323V4AG582DX 1 Rotary Oil Vacuum Pump 115VAC 60Hz
VWR Scientific 0323V4AG21DX 1 Rotary Oil Vacuum Pump 115VAC 60Hz
Cole Parmer P-07055-04 1 Rotary Oil Vacuum Pump 115VAC 60Hz
Cole Parmer P-07055-35 1 Rotary Oil Vacuum Pump 115VAC 60Hz

Suitable Vacuum Bottles and Cap Pairings

Source Part No. Quantity Description
Beckman Coulter, Inc. 975796 1 Heavy-duty Bottle, HDPP 4 Liter
Beckman Coulter, Inc. 975797 1 CAP Filling/Venting 1/2" fittings, PP
VWR Scientific 16331-100 1 Heavy-duty Bottle, HDPP 4 Liter
VWR Scientific 16225-230 1 Filling/Venting CAP 1/2" fittings, PP
Nalgene 2126-4000 1 Heavy-duty Bottle, HDPP 4 Liter
Nalgene 2162-0830 1 Filling/Venting Closure 1/2" fittings, PP
Cole Parmer P-06258-00 1 Filling/Venting CAP 1/2" fittings, PP

* All trademarks are the property of their respective owners. Where applicable, the PCR process is covered by patents owned by Roche Molecular Systems, Inc., and F. Hoffman-LaRoche, Ltd.

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