Beckman Coulter Application (system support only)
Automation of Immunotech's IL-8 ELISA using Beckman Coulter’s Assay Workstation version 1.5
Laura Pajak**, Chad Pittman**, Yu Suen**, and Scott Boyer**
Beckman Coulter, Inc.
**Indianapolis, IN and ***Fullerton, CA
We have developed and optimized an automated method using the Assay WorkStation Version 1.5 to provide a complete, walk-away system that automates ELISAs. The system uses SAMI® WorkStation Scheduling Software to optimize the use of incubations to make processing as efficient as possible. The method allows processing of up to 9 plates in under 7 hours with minimal reagent waste. On-deck plate washing and reading are achieved by using an integrated MW96 washer and AD 340 Automated Labware Positioner (ALP), respectively. Consumables are stored in an automated storage module, Cytomat* Microplate Hotel HS with Plate Shuttle* System and brought onto the worksurface by the Biomek FX Cytomat ALP thereby allowing complete processing without user intervention.
The information provided here will:
• Describe the automated system used to process the ELISA
• Demonstrate the utility of the Assay WorkStation
• Describe the results when processing the Immunotech IL-8 ELISA kit on this system
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