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Automation and Detection of Cisbio’s IP-One HTRF* Assay using Laboratory Automation Workstation and the PARADIGM™ Detection Platform from Beckman Coulter

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Amy Yoder, Keith Roby, James Barry, Laura Pajak, Ph.D, Beckman Coulter, Inc.
Sharon Soh, Chris Harbert, Francois Degorce, Cisbio International

Click to enlarge image
Figure 1. Biomek NX^P workstation with integrated Paradigm Detection Platform.

The IP-One HTRF Assay is a homogeneous competitive immunoassay which can be used to monitor Gq-coupled GPCR activity through the inositol phosphate cascade. Inositol phosphate 1 (IP1) is a downstream metabolite of IP3 which accumulates in cells in the presence of LiCl following Gq-mediated phospholipase C (PLC) activation

Beckman Coulter has worked in collaboration with Cisbio to adapt the IP-One HTRF assay to automation workstations. The assay was automated using the Biomek® NX^P Laboratory Automation Workstation. Detection and analysis was performed using the PARADIGM™ Detection Platform with the Cisbio approved HTRF detection cartridge developed by Beckman Coulter. The target in this study is the muscarinic m1 receptor, overexpressed in a CHO model system, using carbachol and acetylcholine as reference agonists.

This poster describes:

The automated workstations utilized to process the cellular assay.
The results obtained from the HTRF IP-One assay using the PARADIGM Detection Platform and laboratory automation.

Click here for this poster (PPT)

Request More Info on Beckman Coulter Automated Solutions >>

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Additional Information

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Application System Requirements

Hardware Options

Source	Part No.	Quantity	Description
Beckman Coulter, Inc.	A31840	1	Biomek NX^P workstation Span-8 with Gripper
Beckman Coulter, Inc.	989839	1	Biomek NX^P/NX Span-8 4x3 ALP Kit
Beckman Coulter, Inc.	989778	1	Biomek NX^P/NX Half Trash ALP
Beckman Coulter, Inc.	A41572	1	PARADIGM Detection Platform
Beckman Coulter, Inc.	A41573	1	PARADIGM™ HTRF Configuration

Software Options

Source	Part No.	Quantity	Description
Beckman Coulter, Inc.	719349	1	Biomek Software

Consumables and Reagents

Source	Part No.	Quantity	Description
Beckman Coulter, Inc.	379502	1	Tip, P250, Span-8, Sterile, 220 µL (case of 10 racks)
Beckman Coulter, Inc.	373661	1	24-Position Tube Rack
Beckman Coulter, Inc.	356090	1	Tube, Polypropylene, Snap-On Cap, Natural, 1.5 mL, 11 x 38 mm, 0.44 x 1.52 in (pkg of 500)
Beckman Coulter, Inc.	379503	1	Tip, Barrier, P250, Span-8, Sterile, 125 µL (case of 10 racks)
Beckman Coulter, Inc.	A21582	1	P50 Tips, Sterile, Packaged
Beckman Coulter Inc.	372790	1	Quarter Reservoir (case of 40)
Beckman Coulter Inc.	372795	1	Frame for Reservoirs (1 Each)

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* All trademarks are the property of their respective owners. Where applicable, the PCR process is covered by patents owned by Roche Molecular Systems, Inc., and F. Hoffmann-LaRoche, Ltd.

For Research Use Only; not for use in diagnostic procedures.

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