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T³ Update Online -- Tips, Tricks and Techniques for Users of Beckman Coulter Life Science Automation

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Welcome From the Editors – Q2 2009 Issue

In this issue of T3 Update Online, we offer an extensive recap of LabAutomation 2009, which was held in late January in Palm Springs, Calif. The show gave us an opportunity to meet many of you face-to-face, but for those of you who weren’t able to attend, we’ve got you covered—just follow the links to the workshop presentation materials and posters.

We are pleased to have offered five workshop presentations, two of which are included in this issue:

“Cell Culture Automation in Biopharma,” by Dr. Christoph Krüll of Beckman Coulter Europe, introduces automated cell cultivation systems for cell line optimization and monoclonal antibody production from Beckman Coulter.
“Improve Laboratory Processing for Nucleic Acid Analysis, Through Miniaturization and Submicroliter Dispensing,” by Amy Yoder of Beckman Coulter, showcases miniaturized applications on the BioRAPTR FRD™, including PCR, STR (short tandem repeats), cloning and transformation.

Our posters covered topics including:

Beckman Coulter’s partnership with the United States Department of Agriculture (USDA) to create libraries for commercial yeast strains through miniaturization of cloning and transformations;
automation of Promega’s Multitox-Fluor Multiplex Cytotoxicity assay for the breast cancer cell line, MCF7;
automatic cell, compound and reagent dispensing for the AlphaScreen* cAMP assay for the human epithelial carcinoma cell line, A431;
automated dispense of P2RY11 CHO-K1 GPCR cell-based assay for division-arrested cells;
purification of gDNA from whole blood with the Agencourt Genfind™ v2 Purification System using the Biomek® 3000 Laboratory Automation Workstation and SPRI® (Solid Phase Reversible Immobilization) bead technology; and
integrated Biomek workstations that can be incorporated into cultivation processes to provide functionality to streamline automated cell handling.

Marc Goldstein, Ph.D., Mary X. Guan and Keith Roby

Yong Song, Ph.D. and Mary X. Guan, US RAC. Learn more in the "About the Editors" section.

Workshop Presentations

		Cell Culture Automation in Biopharma Christoph Krüll, Ph.D., Beckman Coulter Europe Introducing automated cell cultivation systems for cell line optimization and monoclonal antibody production from Beckman Coulter. Together with process and data management software, these systems provide functionality to streamline automated cell handling, including sample tracking and data management. View the presentation

		Improve Laboratory Processing for Nucleic Acid Analysis, Through Miniaturization and Submicroliter Dispensing Amy Yoder, Beckman Coulter, Inc. Miniaturized applications on the BioRAPTR FRD™are showcased, including PCR, STR (short tandem repeats), cloning and transformation. It covers strategies for miniaturizing an assay, as well as pitfalls and the advantage of using DOE (design of experiment) throughout the miniaturization process. Cost savings of the miniaturized applications covered ranges from 83-98% savings from the full-scale reaction. View the presentation

Posters

		Creating Libraries for Commercial Yeast Strains through Miniaturization of Cloning and Transformations Using the BioRAPTR FRD™ Microfluidic Workstation Amy Yoder, Laura Pajak, Ph.D. Beckman Coulter Inc John S. Jackson, M.S., Stephen R. Hughes, Ph.D. USDA, ARS, National Center for Agricultural Utilization Research, Bioproducts and Biocatalysis Group View the poster

		MultiTox-Fluor Multiplex Cytotoxicity Assay for the Breast Cancer Cell line, MCF7, Using the BioRAPTR FRD™ Microfluidic Workstation and PARADIGM™ Detection Platform Li Liu Ph.D. and Laura Pajak Ph.D., Beckman Coulter, Inc. View the poster

		*AlphaScreen cAMP Assay for the Human Epithelial Carcinoma Cell Line, A431, Using the BioRAPTR FRD™ Microfluidic Workstation and PARADIGM™ Detection Platform** Li Liu Ph.D. and Laura Pajak Ph.D., Beckman Coulter, Inc. View the poster

		*GeneBLAzer P2RY11 CHO-K1 GPCR Cell-Based Assay for Division-Arrested Cells, Using the BioRAPTR FRD™ Microfluidic Workstation and PARADIGM™ Detection Platform** Ruth Zhang Ph.D. and Laura Pajak Ph.D., Beckman Coulter, Inc. View the poster

		Purification of Genomic DNA from Whole Blood With the Agencourt® Genfind™ v2 Purification System Using the Biomek® 3000 Laboratory Automation Workstation Ruth Zhang, Ph.D., Li Liu, Ph.D., Mary Blair, Laura Pajak, Ph.D., Beckman Coulter, Inc. View the poster

		Automating Cell Culture Process with Integrated Biomek® Workstations Bryan Davis, Robert Lund, Jesseca Lyons, Ron Shipman, Yong Song Life Science Automation, Beckman Coulter, Inc. View the poster