HematoFlow Technology with CytoDiff 

HematoFlow with CytoDiff* enables laboratories to shift a significant portion of the manual slide review burden to an automated instrument. This allows labs to receive results faster, while enabling staff to perform automated analyses without the years of staff experience and training required for manual reviews. The system also offers enhanced differential results and standardization across the laboratory, providing additional clinical information and improving patient management.

How CytoDiff Implementation Can Reduce Your Manual Review Rate to 5%


Before CytoDiff* implementation, all samples with flags for WBC abnormalities undergo manual review. Download the PDF to see how, after CytoDiff* implementation, 95% of samples can be analyzed with an automated system (see Figure 1).


HematoFlow is our exclusive technology that links hematology and flow cytometry with a multi-color monoclonal antibody solution. It provides automated, accurate and detailed differential information to aid physicians in the diagnosis and treatment of patients with abnormal WBC test results. With HematoFlow, WBC differentials go beyond traditional cellular analysis, enabling laboratories to produce results more quickly with a reduced need for manual review. 


CytoDiff software applies proprietary algorithms to automatically separate and detail cell populations using a reagent cocktail composed of six monoclonal antibodies in five colors. The result is an automated 10-part cytometric WBC differential—the traditional five-part WBC differential with additional immunologic subpopulations of B and T/NK lymphocytes, as well as abnormal populations of immature granulocytes and blasts. 

How HematoFlow with CytoDiff Delivers Better Reviews Faster

  • Enhanced clinical utility. The automated 10-part differential provides far greater insights than those available from a traditional complete blood cell count 
  • Improved sensitivity and statistical accuracy. HematoFlow with CytoDiff analyzes a much larger number of cells—20,000 vs. 100 to 200 in a manual slide review—making it ideal for low white blood cell populations 
  • Improved standardization. Increased precision eliminates technician-to-technician variability within the laboratory and enables reproducible charts and graphs 
  • Significantly reduced manual review rates. Laboratories can reduce the need for manual review by up to eight times, eliminating unnecessary slide making, staining, drying and microscopic examination1-6 
  • Simplified usage. HematoFlow with CytoDiff analysis can be learned in two to three days, without previous experience in flow cytometry, versus years of practice required for effective microscopic review 

Bibliography for CytoDiff References

HematoFlow with CytoDiff has been featured in numerous peer-reviewed articles investigating the clinical and workflow advantages of the system compared to traditional white blood cell differential. For more information on examples by customers, download our bibliography for CytoDiff references.
1Roussel, Mikael et al. “Refining the white blood cell differential: The first flow cytometry routine application.” Cytometry Part A, Clinical Cytometry, 2010 June;77A(6):552-563. 
2Jo, Yongjun et al. “Reliable, accurate determination of the leukocyte differential of leukopenic samples by using Hematoflow method.” Korean Journal of Laboratory Medicine, 2011 July;31(3):131-137. 
3Gac, F. et al. “Evaluation of CytoDiff™ on cord blood WBC differential.” International Journal of Laboratory Hematology, 2013;35(1):46-54. 
4Kim, Ah Hyun et al. “White Blood Cell Differential Counts in Severely Leukopenic Samples: A Comparative Analysis of Different Solutions Available in Modern Laboratory Hematology.” Blood Research, 2014;49(2):120-126. 
5Allou, Kaoutar et al. “The routine leukocyte differential flow cytometry HematoFlow™ method: A new flagging system for automatic validation.” Cytometry Part B, Clinical Cytometry, 2015;88(6):375-84. 
6Kahng, Jimin et al. “Flow Cytometric White Blood Cell Differential Using CytoDiff Is Excellent for Counting Blasts.” Annals of Laboratory Medicine, 2015;35(1):28-34.  
*HematoFlow and CytoDiff are not available for sale in the U.S.