Access BNP & NT-proBNP Immunoassays

Natriuretic peptides reflect cardiac status and effects of therapy

Access BNP and NT-proBNP immunoassays are used to aid in the diagnosis of heart failure and left ventricular dysfunction.¹
B‐type natriuretic peptide (BNP) is a cardiac hormone originating in the heart.² In patients with heart failure, BNP and NT-proBNP levels are elevated.³ They can be assessed as important measures of cardiac function and diagnosis of heart failure.⁴

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Efficiently support diagnosis and assessment of heart failure severity with trustworthy results

B-type natriuretic peptide (BNP) and the N-terminal pro hormone BNP (NT-proBNP) are well established for use in diagnosis of heart failure. Following production, the prohormone is cleaved at a 1:1 ratio into NT-proBNP and BNP. (Fig. 1) BNP is involved in regulating blood volume.² Blood levels of NT-proBNP and BNP increase when the left ventricle is stretched, as occurs during heart failure, a condition where the heart cannot pump enough blood.

Natriuretic peptides play a significant role in controlling cardiovascular function. The biologically active BNP ring is involved in cardiorenal homeostasis by stimulating diuresis and natriuresis. Both NT-proBNP and BNP biomarkers provide demonstrated benefit for heart failure patients in emergency and outpatient settings. Both NT-proBNP and BNP are included in standard of care guidelines for both the American College of Cardiology/American Heart Association⁵ and European Society of Cardiology.⁶

Laboratories can help clinicians deliver patient care with confidence for all stages of heart failure with Access NT-proBNP and BNP assays, highly sensitive and specific assays that aid clinicians in heart failure diagnosis, assessment of severity, and risk stratification.

Access BNP Cardiac
Figure 1. BNP and NT-proBNP production in the ventricular cardiac myocardium⁷

NT-proBNP

Increase accuracy in diagnosing heart failure with age-based cutoffs that improve specificity by 31% compared with a single cutoff strategy and specific information that supports interpretation of results in patients with comorbidities.

Results in under 11 minutes
Limit of Detection (LoD) ≤10 ng/L
Sample stability for 72 hours at room temperature
Compatible with serum, LiHep and EDTA plasma

BNP

Advance patient care with a strong predictor of clinical outcomes that supports diagnosis and assessment of severity for congestive heart failure and risk stratification of those with acute coronary syndrome.

Measures active hormone
General specificity of 98% using a threshold of 100 pg/mL
Total observed imprecision <7%
Analytical sensitivity: 1 pg/mL (95% confidence)

The Access NT-proBNP and BNP assays are not intended to be used in isolation; results should be interpreted in conjunction with other diagnostic tests and clinical information.

	BNP	NT-proBNP
Analytical Measuring Range	1 to 5000 pg/mL	10 to 35,000 ng/L (pg/mL)
Sample Type/Size	Plasma (EDTA)	Serum, lithium heparin plasma, and EDTA
Time to First Result	≥16.2 minutes	<11 minutes
Analytical Sensitivity	1 pg/mL (95% confidence)	Limit of Detection (LoD) ≤10 ng/L (pg/mL) 20% CV Limit of Quantitation (LoQ) ≤10 ng/L (pg/mL)
Calibrator Levels	0, and approximately 25, 100, 500, 2500, and 5000 pg/mL	0, and approximately 25, 100, 500, 2500, and 5000 pg/mL
Open pack stability	28 days	61 days
Calibration stability	28 days	28 days
Total Imprecision	<7%	≤4.0 ng/L (pg/mL) SD at concentrations ≤50 ng/L (pg/mL) ≤8.0% CV at concentrations >50 ng/L (pg/mL)

BNP & NT-proBNP Technical Bulletins

Triage BNP Technical Bulletin - Biotin Interference
This important information pertains to the Quidel Triage BNP Test for the Beckman Coulter® Access Family of Immunoassay Systems (Triage BNP for BCIS).

Access BNP Technical Bulletin – Biotin Interference
This important information pertains to the Access BNP test for the Access Family of Immunoassay Systems

View all documents

Multidisciplinary Views from the Laboratory, Emergency Department,
and Cardiology

Speakers:

Robert Christenson, PhD, DABCC, FAACC, FACC
Professor of Pathology & Professor of Medical and Research Technology
University of Maryland School of Medicine

W. Frank Peacock IV, MD, FACEP, FACC, FESC
Professor of Emergency Medicine and Vice Chair of Research for the Department of Emergency Medicine
Baylor College of Medicine

James Januzzi, MD
Cardiologist and Director, Dennis and Marilyn
Barry Fellowship in Cardiology Research Massachusetts General Hospital

Natriuretic Peptides 101: Perspectives from the Laboratory and Emergency Department

Hear from Drs. Robert Christenson and W. Frank Peacock on the value of BNP and NT-proBNP testing.

Video

Key considerations when deciding between BNP and NT-proBNP

Video

Importance of turnaround time for cardiac STAT assays

Video

Considerations for interpreting natriuretic peptide values

Video

Importance of reference intervals

Video

Considerations for comorbidities when interpreting natriuretic peptide results

Video

The future of heart failure diagnostics

Video

The impact of clinical decision support and artificial intelligence-related technologies on heart failure risk assessment

Interested in learning more? Watch the full webinar.

Watch now

Natriuretic Peptides 201: Perspectives from the Laboratory and Cardiology

Hear from Drs. Robert Christenson and James Januzzi on the value of BNP and NT-proBNP in heart failure diagnostics and therapeutic monitoring.

Natriuretic peptide assays use in clinical practice

Diagnosis of heart failure in the office

Other presentations for guiding natriuretic peptide use

Implications of differing natriuretic peptide test results

Benefits and differences of age-related cutoffs for natriuretic peptides

Clinician complaints about natriuretic peptide assays

Future of heart failure diagnostics and natriuretic peptides in 10 years

Interested in learning more? Watch the full webinar.

Watch now

References:

1. Bionda C, Bergerot C, Ardail D, Rodriguez-Lafrasse C, Rousson R. Plasma BNP and NT-proBNP assays by automated immunoanalyzers: analytical and clinical study. Ann Clin Lab Sci. 2006;36(3):299-306.

2. Weber M, Hamm C. Role of B-type natriuretic peptide (BNP) and NT-proBNP in clinical routine. Heart. 2006;92(6):843-849. doi:10.1136/hrt.2005.071233

3. Yancy CW, Jessup M, Bozkurt B, et al. 2013 ACCF/AHA guideline for the management of heart failure: a report of the American College of Cardiology Foundation/American Heart Association Task Force on Practice Guidelines. J Am Coll Cardiol. 2013;62(16):e147-239. doi:10.1016/j.jacc.2013.05.019

4. Chien T-I, Chen H-H, Kao J-T. Comparison of Abbott AxSYM and Roche Elecsys 2010 for measurement of BNP and NT-proBNP. Clin Chim Acta. 2006;369(1):95-99. doi:10.1016/j.cca.2006.01.017

5. Heidenreich PA, Bozkurt B, Aguilar D, et al. 2022 AHA/ACC/HFSA guideline for the management of heart failure: executive summary: A report of the American College of Cardiology/American Heart Association Joint Committee on Clinical Practice Guidelines. Circulation. 2022;145(18):e876-e894. doi:10.1161/CIR.0000000000001062

6. McDonagh TA, Metra M, Adamo M, et al. 2021 ESC Guidelines for the diagnosis and treatment of acute and chronic heart failure. Eur Heart J. 2021;42(36):3599-3726. doi:10.1093/eurheartj/ehab368

7. Kim YS, Karisa N, Jeon WY, Lee H, Kim Y-C, Ahn J. High-level production of N-terminal pro-brain natriuretic peptide, as a calibrant of heart failure diagnosis, in Escherichia coli. Appl Microbiol Biotechnol. 2019;103(12):4779-4788. doi:10.1007/s00253-019-09826-8

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Access BNP & NT-proBNP Immunoassays

Efficiently support diagnosis and assessment of heart failure severity with trustworthy results

Analytical Measuring Range

Sample Type/Size

Time to First Result

Analytical Sensitivity

Calibrator Levels

Open pack stability

Calibration stability

Total Imprecision

BNP & NT-proBNP Technical Bulletins

Multidisciplinary Views from the Laboratory, Emergency Department, and Cardiology

Speakers:

Natriuretic Peptides 101: Perspectives from the Laboratory and Emergency Department

Interested in learning more? Watch the full webinar.

Natriuretic Peptides 201: Perspectives from the Laboratory and Cardiology

Interested in learning more? Watch the full webinar.

Immunoassay Analyzers

Access 2 Analyzer

DxI 600 Analyzer

DxI 800 Analyzer

DxI 9000 Analyzer

Multidisciplinary Views from the Laboratory, Emergency Department,
and Cardiology