Study establishes pediatric reference intervals for 47 hematologic parameters


In a study published in American Journal of Clinical Pathology, fresh whole blood samples were collected from 566 healthy children and adolescents (birth to <21 years). The samples were analyzed for 47 hematologic parameters on the Beckman Coulter DxH 900. Age- and sex-specific reference standards were calculated based on the Clinical and Laboratory Standards Institute guidelines.

The study’s findings:

  • Established a robust database of pediatric reference standards for 47 hematologic parameters in the Canadian Laboratory Initiative on Pediatric Reference Intervals (CALIPER) cohort for the first time
  • Along with accompanying publication containing hematologic Reference Intervals (RIs) on the Beckman Coulter DxH 9001, have the potential to address gaps in pediatric RIs for hematologic parameters and, as a result, improve the accuracy of laboratory result interpretation and clinical decision making in children and adolescents

At a Glance

Healthy children and adolescents (birth to <21 years)
Hematologic parameters were established with age- and sex-specific reference intervals
1 st
Comprehensive CALIPER study to establish hematologic RIs on the DxH 900, as well as develop RIs for emerging hematologic biomarkers, including the novel early sepsis indicator, MDW

Source: Tahmasebi, H., Higgins, V., Bohn, M. K., Hall, A., & Adeli, K. (2020, June 20). CALIPER Hematology Reference Standards (I): Improving Laboratory Test Interpretation in Children (Beckman Coulter DxH 900–Core Laboratory Hematology System). Oxford Academic, American Journal of Clinical Pathology.


1. Higgins V, Tahmasebi H, Bohn MK, et al. CALIPER hematology reference standards (II): improving laboratory test interpretation in children (Beckman Coulter DxH 520–Physician Office Hematology System) with analytical comparison to the Beckman Coulter DxH 900. Am J Clin Pathol. 2020; DOI: 10.1093/ajcp/aqaa057.