Study validates development of cost-effective semi-quantitative diagnostic method with 90% sensitivity

Study validates semi-quantitative diagnostic method with 90% sensitivity

In a study published in Journal of Blood Medicine, researchers analyzed 179 convalescent plasma units for the presence of SARS-CoV-2 IgG antibodies. This study was conducted to determine if an automated chemiluminescent SARS-CoV-2 IgG antibody assay platform can accurately quantify the number of binding antibodies present in donated convalescent plasma.

The study concluded:

  • The utility of automated antibody detection systems can be extended from simply a screening method to a semi-quantitative and quantitative functional antibody analysis
  • Chemiluminescent immunoassay equipment-derived numerical values can be used to reliably estimate the ELISA antibody titer
  • There was significant linear correlation between the CLIA S/Co values and ELISA IgG titers. This indicates that comparable antibody quantification can be reliably derived from the results of an automated antibody detection system
  • Incorporation of chemiluminescent-based methods can provide rapid, cost-effective means of identifying anti-SARS-CoV-2 antibody titers in donated plasma for use in the treatment of COVID-19 infection

At a Glance

179
Convalescent plasma units analyzed for the presence of SARS-CoV-2 IgG antibodies
90 %
Sensitivity in predicting a titer dilution of ≥1:1024 with an identified CLIA S/Co cutoff value of 8.2
82 %
Specificity in predicting a titer dilution of ≥1:1024 with an identified CLIA S/Co cutoff value of 8.2

Source: Mendoza, R., Silver, M., Zuretti, A., Christian, M., Das, B., Norin, A., Borgen, P., Libien, J., & Bluth, M. (2021). Correlation of Automated Chemiluminescent Method with Enzyme-Linked Immunosorbent Assay (ELISA) Antibody Titers in Convalescent COVID-19 Plasma Samples: Development of Rapid, Cost-Effective Semi-Quantitative Diagnostic Methods. Journal of Blood Medicine, Volume 12, 157–164. https://doi.org/10.2147/jbm.s296730