The PK7400 Automated Microplate System delivers the industry’s highest throughput and improves workflow efficiency through fully automated, batch-test functionality. The next evolution in PK innovation, the PK7400 donor testing analyzer, enables you to optimize testing using proven agglutination technology and a comprehensive blood typing menu.
Experience the highest throughput of reliable blood donor immunohematology testing with this blood banking analyzer.
Endless Benefits
The PK7400 automated blood banking analyzer delivers unmatched throughput.
Simple to Use Donor Testing Analysis
The PK7400 Donor Testing Analyzer Delivers Peace of Mind
| General Characteristics | |
| Analytical method | Agglutination method on terraced microplates |
| Channels | 12 |
| Throughput | 300 samples/hour with 5 diluted sample cups |
| Sample capacity | 12 racks or 120 samples; continuous sample-rack loading allowed |
| Sample tube size | In primary or secondary tubes: diameter: 12‒15 mm; height: 75‒100 mm |
| Sample | Plasma, serum, red blood cells |
| Reagent tray | Up to 16 reagents can be loaded; 12 positions for primary reagents (R1); 4 positions for secondary reagents (R2) |
| Reaction vessel | Terraced microplates |
| Reaction time | 60 minutes |
| Assays | ABO blood grouping, Rh typing, including weak D testing, red blood cell antigen screening and syphilis qualitative screeningⱡ |
| Sample barcode | NW-7, CODE 39, CODE 128, ISBT-CODE 128, 2 of 5 Interleaved and EAN-13 |
| Analyzer dimensions | 1,760 mm (W) x 920 mm (D) x 1,380 mm (H) / 69 in (W) x 36 in (D) x 54 in (H) |
| Console dimensions | Console dimensions 800 mm (W) x 720 mm (D) x 1,450 mm (H) / 32 in (W) x 28 in (D) x 57 in (H) |
| Weight | 750 kg / 1,653 lbs |
| Electrical Requirements | |
| Electrical consumption | 3.0 kVA maximum |
| Current | 15‒30 amp (with UPS) |
| Circuit | Dedicated and noise-free |
| Voltage | 200/208/220/230/240 VAC (±10%) single phase |
| Frequency | 50/60 Hz (±1 Hz) |
| Ground requirement | <100 ohms |
| Location | Power cable length 10 m (30 ft) |
| Water Requirements | |
| Type | Deionized |
| Supply | Continuous flow |
| Resistivity | >0.5 mega ohms |
| Mechanical filtration | <0.5 µm (glycerol-free) filter at discharge of deionizer |
| Consumption | 50 L/hour max, instantaneous demand 3.5 L/minute |
| Pressure | 0.49 x 105 Pa to 3.92 x 105 Pa (7.1‒58.8 PSI) |
| Location | Shutoff valve within 10 m (30 ft) |
| Tubing diameter | 12 mm (ID) x 18 mm (OD) |
| Connection | 0.5 in barbed-hose fitting |
| Drain Requirements | |
| Gravity | Hazardous waste floor drain: maximum height 1.5 m (5 ft); max distance from analyzer: 10 m (30 ft) |
| Tubing requirements | 15 mm (ID) x 22 mm (OD) |
| Environmental Requirements | |
| Average heat output | 7,200 KJ/H (6,824 BTU) max |
| Ambient temperature | 18–28°C (fluctuations during measurement shall be within ± 2°C) |
| Ambient humidity | 20%–80% relative humidity |
| Noise output | Max 65 dB or less |
Beckman Coulter supports you with a full complement of accessories and expendable parts for the PK7400 System. Beckman Coulter offers training to end users on proper use and replacement of all accessories and expendable parts. The replacement of consumable parts can be monitored in an online menu located on the PK7400. This menu can serve as a tool for establishing a time line for purchasing consumable parts.
The PK Blood Grouping Reagents are intended for the determination of ABO blood group, Rh and Kell phenotypes in blood donors using Beckman Coulter PK Systems.
The Anti-A, Anti-B and Anti-A,B reagents are used in the red blood cell determination of the ABO blood group. They are used to determine the absence or presence of erythrocytic antigens A and/or B on the surface of human red blood cells.
The Anti-D reagents: Anti-D, Anti-D (PK1) and Anti-D (PK2) are used to determine the Rh type, to detect the presence of the D (Rh) antigen on the surface of human red blood cells. The Anti-C, Anti-E, Anti-c̄, Anti-ē and Anti-K are used for Rh-Kell phenotyping of human red blood cells. These reagents detect the presence of antigens C, E, c̄, ē and K on the surface of red blood cells.
The PK Control is devoid of antibody activity and should be tested in parallel with the PK System Blood Grouping and Phenotyping reagents to differentiate between specific and non-specific agglutination.
| Sample | Sample Expiration | Storage Condition |
| EDTA | 5-day sample age | 2-8°C |
PK System Reagent Red Blood Cells (A1 and B) are intended for the determination of the reverse or plasma group on Beckman Coulter PK systems.
The determination of an ABO blood group is defined by demonstrating the presence or absence of A and/or B on the surface of human red blood cells and detecting the presence or absence of Anti-A and/or Anti-B antibodies in the plasma. The red blood cell antigens are identified using the Beckman Coulter PK System blood grouping reagents. Anti-A, Anti-B and Anti-AB (red blood cell or forward group). The PK System Reagent Cells A1 and B (plasma or reverse group) confirm the red blood cell group by verifying the presence of the corresponding antibodies in the plasma by using known red blood cells.
| Sample | Sample Expiration | Storage Condition |
|---|---|---|
| EDTA | 5-day sample age | Age 2–8°C |
| Reagents and Sample Diluent | Part Number | Kit |
| Anti-A | 17301 | 10 x 20 mL |
| Anti-A | 27301 | 10 x 20 mL |
| Anti-B | 17302 | 10 x 20 mL |
| Anti-B | 27302 | 10 x 20 mL |
| Anti-A,B | 17303 | 10 x 20 mL |
| Anti-D | 17304 | 10 x 20 mL |
| Anti-D (PK1) | 17305 | 10 x 20 mL |
| Anti-D (PK2) | 17306 | 10 x 20 mL |
| Control | 17317 | 10 x 20 mL |
| Reagent Red Blood Cells A1 and B | 17318 | 2 x 2 x 20 mL |
| Anti-C | 17309 | 1 x 20 mL |
| Anti-E | 17307 | 1 x 20 mL |
| Anti-c̄ | 17314 | 1 x 20 mL |
| Anti-ē (PK7300 only) | 17319 | 1 x 20 mL |
| Anti-ē (NEW-PK7400) | B49558 | 1 x 20 mL |
| Anti-K | 17315 | 1 x 20 mL |
| Bromeline | 17330 | 6 x 900 mL |
The PK7400 TP HA reagent is intended for the qualitative screening of blood and plasma donors for the detection of Treponema pallidum IgG and IgM antibodies to syphilis in human serum, EDTA plasma or CPDA plasma using the PK7400 System. This assay is not intended for diagnostic use. The ready-to-use reagents are uniform, stable and easy to handle, which reduces preparation error and labor needed to perform the assay.
| Sample Type | Sample Expiration | Storage Condition |
| EDTA |
7-day sample age |
2–8°C |
| Serum |
7-day Sample age |
2–8°C |
Conveniently packaged with:
| Product | Part Number |
Kit |
| PK7400 TP HA Reagent |
B11186 |
3 x 45 mL |
| PK7400 TP HA Controls |
B11187 |
Reactive: 8 x 5 mL Nonreactive: 8 x 5 mL |
The PK CMV-PA System is a passive particle agglutination assay intended for the qualitative detection of IgG and IgM antibodies to cytomegalovirus (CMV) in human plasma and serum from blood donors using Beckman Coulter PK Systems. This test is not intended for diagnostic use. The PK CMV-PA System has been developed to provide an indirect particle agglutination CMV assay using uniform reagents, which are stable and easy to handle.
| Sample | Sample Expiration | Storage Condition |
| EDTA | 5-day sample age | 2–8°C |
| Serum | 14-day sample age | 2–8°C |
| Product | Part Number | Kit |
| PK CMV-PA System | PA2300 | 10 x 6 mL |
| PK CMV-PA System Controls | PA2302 | Reactive 6 x 4 mL Nonreactive 6 x 4 mL |
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When performing analysis on PK Systems, it is important to compare the data and the image for every sample tested to obtain accurate results. Learn how to troubleshoot abnormal images, specifically fold-over in the PK Chat.
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The PK7400 Automated Microplate System delivers the industry’s highest throughput and improves workflow efficiency through fully automated, batch-test functionality.
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1. World Health Organization. Blood safety and availability. June 10, 2020. Access Oct. 19, 2021. https://www.who.int/news-room/fact-sheets/detail/blood-safety-and-availability
2. American Red Cross Blood Services. US Blood Supply Facts. Importance of Blood Supply. Retrieved Oct. 19, 2021. https://www.redcrossblood.org/donate-blood/how-to-donate/how-blood-donations-help/blood-needs-blood-supply.html
3. American Red Cross Blood Services. US Blood Supply Facts. Importance of Blood Supply. Retrieved Oct. 19, 2021. https://www.redcrossblood.org/donate-blood/how-to-donate/how-blood-donations-help/blood-needs-blood-supply.html
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