Benefits of the Access Active-B12 assay
- Market-leading time to first result, the widest measuring range on the market and longer time between calibrations to bring efficiency to laboratory workflow
- Standardization to WHO IS 03/178, providing accurate and harmonized results
- Improved diagnostic accuracy for vitamin B12 deficiency, with fewer indeterminate results than serum vitamin B12 tests1
- Confidence in results with no interference from intrinsic factor antibodies
Vitamin B12 and its impact on patient health
Vitamin B12 deficiency can have a profound impact on a person's health. Among numerous consequences is megaloblastic anaemia and progressive neurologic disease of the central and peripheral nervous system.2 Vitamin B12 (cobalamin) is an essential nutrient naturally found in meat, fish and dairy products and can only be obtained through dietary intake. B12 deficiency can be prevalent in individuals who consume no animal-sourced foods or vegetarians who eat too little egg or dairy products. The condition is also prevalent in the elderly, where conditions such as atrophic gastritis, pernicious anaemia, Crohn’s disease and immune system disorders can impact how the body absorbs vitamin B12.
Vitamin B12 in circulation exists in two forms: holotranscobalamin (holoTC) and holohaptocorrin (holoHC). Only holoTC transports vitamin B12 from its site of absorption in the ileum to tissues and cells throughout the body, while holoHC is considered inert or biologically unavailable. HoloTC represents 10–30% of the total circulating B12 and is considered the bioactive form of B12 or Active-B12.2 While traditional serum B12 testing measures the entirety of circulating B12, only the Active-B12 assay measures the critical form. Evidence suggests that when used as either a screening or resolving test, Active-B12 provides a more reliable and earlier indicator of B12 deficiency. It also ensures fewer indeterminate results, as well as greater sensitivity and specificity than total serum B12 or methylmalonic acid tests.1,3