Access Active-B12 Assay

Greater confidence in vitamin B12 test results

The Access Active-B12 assay is the newest addition to an already comprehensive menu for anemia management. With the largest measuring range on the market, fastest time to first result and standardization to WHO International Standard (IS) 03/178, Access Active-B12 delivers confidence and flexibility over other methods of vitamin B12 testing.

This product may not be available in your country or region at this time. Please contact your Beckman Coulter sales representative or distributor for more information.

Benefits of the Access Active-B12 assay

  • Market-leading time to first result, the widest measuring range on the market and longer time between calibrations to bring efficiency to laboratory workflow
  • Standardization to WHO IS 03/178, providing accurate and harmonized results
  • Improved diagnostic accuracy for vitamin B12 deficiency, with fewer indeterminate results than serum vitamin B12 tests1
  • Confidence in results with no interference from intrinsic factor antibodies

Vitamin B12 and its impact on patient health

Vitamin B12 deficiency can have a profound impact on a person's health. Among numerous consequences is megaloblastic anaemia and progressive neurologic disease of the central and peripheral nervous system.2 Vitamin B12 (cobalamin) is an essential nutrient naturally found in meat, fish and dairy products and can only be obtained through dietary intake. B12 deficiency can be prevalent in individuals who consume no animal-sourced foods or vegetarians who eat too little egg or dairy products. The condition is also prevalent in the elderly, where conditions such as atrophic gastritis, pernicious anaemia, Crohn’s disease and immune system disorders can impact how the body absorbs vitamin B12.

Vitamin B12 in circulation exists in two forms: holotranscobalamin (holoTC) and holohaptocorrin (holoHC). Only holoTC transports vitamin B12 from its site of absorption in the ileum to tissues and cells throughout the body, while holoHC is considered inert or biologically unavailable. HoloTC is considered the bioactive form of B12 or Active-B12.2 While traditional serum B12 testing measures the entirety of circulating B12, only the Active-B12 assay measures the critical form. Evidence suggests that when used as either a screening or resolving test, Active-B12 provides a more reliable and earlier indicator of B12 deficiency. It also ensures fewer indeterminate results, as well as greater sensitivity and specificity than total serum B12 or methylmalonic acid tests.1,3


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Happy and healthy older couple enjoying life without the worries of anemia

Article Anemia: Finding Causes, Supporting Treatment

Active-B12 assessment is one of many tools that can be used to help diagnose and treat anemia. Anemia is a common disease that leads to a significant burden on healthcare. Find out about the serious economic and quality-of-life impacts of anemia, as well as how a comprehensive suite of anemia screening and diagnostic tools can help your laboratory support patients experiencing or at risk for this condition.

Learn more

1Valente E, et al, Clin Chem, 2011;57:6 856–863.

2Brady J, et al, Clin Chem, 2008: 54;3 567–573.

3Herrman W, Obeid RVakur Bor M, Nexo E. "Vitamin B12—cobalamin". In: Herrman W, Obeid R, eds. Vitamins in the prevention of human diseases. Berlin: De Gruyter, 2011:187–99.